Method for detecting in-vitro stability of siRNA (small interfering ribonucleic acid)

The invention belongs to a detection method in the field of gene therapy, and particularly relates to a method for treating siRNA (small interfering Ribonucleic Acid) by using animal liver homogenate and detecting the stability of the siRNA by using non-denaturing gel. The method comprises an in-vit...

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Hauptverfasser:	ZHANG RUI, XIAO PUHAO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to a detection method in the field of gene therapy, and particularly relates to a method for treating siRNA (small interfering Ribonucleic Acid) by using animal liver homogenate and detecting the stability of the siRNA by using non-denaturing gel. The method comprises an in-vitro siRNA degradation system and an in-vitro siRNA stability detection system. 本发明属于基因治疗领域的检测方法，特别是一种利用动物肝匀浆处理siRNA并用非变性凝胶检测siRNA稳定性的方法。该方法包括体外降解siRNA体系和体外检测siRNA稳定性系统。