Construction method and application of VSIG4 gene humanized non-human animal

The invention provides a humanized VSIG4 protein, a humanized VSIG4 gene, a targeting vector of the VSIG4 gene, a construction method of a non-human animal subjected to humanized modification of the VSIG4 gene, the humanized non-human animal and application of the humanized non-human animal in the field of biological medicine. A nucleotide sequence for coding the human VSIG4 protein is introduced into a non-human animal genome in a homologous recombination mode, the human or humanized VSIG4 protein can be normally expressed in an animal body, and the human VSIG4 protein can be used as an animal model for human VSIG4 signal mechanism research and tumor and immune disease drug screening, and has important application value for new drug research and development of immune targets. 本发明提供了一种人源化VSIG4蛋白、一种人源化VSIG4基因、一种VSIG4基因的靶向载体、一种VSIG4基因人源化改造非人动物的构建方法、一种人源化的非人动物和其在生物医药领域的应用，利用同源重组的方式将编码人VSIG4蛋白的核苷酸序列导入非人动物基因组中，该动物体内能正常表达人或人源化VSIG4蛋白，可以作为人VSIG4信号机理研究、肿瘤及免疫性疾病药物筛选的动物模型，对免疫靶点的新药研发具有重要的应用价值。