Method for cultivating high-resistance starch indica rice variety by editing rice SSIIIa gene

The invention discloses a method for cultivating a high-resistance starch indica rice variety by editing a rice SSIIIa gene. According to the method, the SSIIIa gene of rice is edited and knocked out, so that the expression quantity of the SSIIIa gene of the rice is reduced, and the high-resistance starch indica rice variety is obtained. The rice starch synthase SSIIIa gene is subjected to fixed-point editing by applying a CRISPR-CAS9 technology, the indica rice variety with high resistant starch content and high amylose content is bred, the yield is high, rice blast resistance is achieved, the requirement of diabetics for rice with high resistant starch content is met, the method is simple, the breeding period is short, and the breeding cost is low. The breeding efficiency can be greatly improved. 本发明公开了一种编辑水稻SSIIIa基因培育高抗性淀粉籼稻品种的方法。是对水稻的SSIIIa基因进行编辑敲除，使的水稻SSIIIa基因表达量降低，获得高抗性淀粉籼稻品种。本发明提供的一种应用CRISPR-CAS9技术对水稻淀粉合成酶SSIIIa基因进行定点编辑，选育出高抗性淀粉含量、高直链淀粉含量的籼稻品种，产量高，抗稻瘟病，以满足糖尿病人对高抗性淀粉含量稻米的需求，本发明方法简单，育种周期短，能够大大提高了育种效率。