Serum albumin combined nano antibody as well as preparation method and application thereof

The embodiment of the invention discloses a serum albumin binding nano-antibody and a preparation method and application thereof.The serum albumin binding nano-antibody comprises a nano-antibody Nb8, and the amino acid sequence of the nano-antibody Nb8 is shown as Seq. ID No.1. The nano-antibody Nb8 has high affinity to bovine serum albumin and can be used for preparing the serum albumin binding nano-antibody. Meanwhile, the compound has relatively good binding activity to mouse serum albumin and human serum albumin, has a relatively good broad-spectrum application range, and can be effectively applied to preparation of drugs for enhancing the half-life period of the drugs and preparation of tumor cell imaging reagents. 本发明实施例公开了一种血清白蛋白结合纳米抗体及其制备方法与应用，其中，血清白蛋白结合纳米抗体包括纳米抗体Nb8，其氨基酸序列如Seq.ID No.1所示，纳米抗体Nb8不仅对牛血清白蛋白具有较高的亲和力，同时对小鼠血清白蛋白和人血清白蛋白均有较好的结合活性，具有较好的广谱使用范围，能够有效应用于制备增强药物半衰期的药物中以及制备肿瘤细胞成像的试剂中。