Protein liquid-liquid phase separation condition screening kit based on biomacromolecule crowding agent and use method

The invention relates to a protein liquid-liquid phase separation condition screening kit based on a biomacromolecule crowding agent and a use method thereof, the biomacromolecule crowding agent is used as a key component and is matched with other additives to prepare a reagent mixture, and the protein liquid-liquid phase separation condition screening kit with 96 reagent conditions is formed. When in use, a target protein is dissolved in ultrapure water to prepare a protein solution, the protein solution is respectively mixed with each screening reagent in the kit according to a certain volume ratio to obtain a protein phase separation condition screening array, the protein phase separation condition screening array is put into a temperature control box to be incubated for a certain time and then taken out to be observed, and when a phase separation phenomenon occurs, the protein phase separation condition screening array is obtained. The corresponding screening reagent is the chemical condition capable of p