Method for detecting polyphenol compounds in yellow rice wine
The invention discloses a method for detecting polyphenol compounds in yellow wine, which comprises the following steps: (1) preparing a polyphenol compound mixed standard working solution, detecting by adopting a liquid chromatograph-mass spectrometer, fitting a standard curve, and calculating a de...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a method for detecting polyphenol compounds in yellow wine, which comprises the following steps: (1) preparing a polyphenol compound mixed standard working solution, detecting by adopting a liquid chromatograph-mass spectrometer, fitting a standard curve, and calculating a detection limit and a quantitation limit; (2) sample pretreatment: a, adjusting the pH value of a wine sample to 2.0 by concentrated hydrochloric acid; extracting the sample with ethyl acetate, centrifuging, repeating once, and combining the supernate; b, re-extracting with diethyl ether, centrifuging, repeating for one time, and combining supernate; c, concentrating the supernate sample to be nearly dry through a vacuum parallel concentrator, dissolving residues in an acetonitrile water solution, and filtering the membrane to obtain a sample to be detected; and (3) detecting on a machine by using a liquid chromatography-mass spectrometry method. The invention establishes a simple, rapid, accurate and high-sensitivit |
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