Method for detecting content of recombinant human erythropoietin

The invention relates to a method for detecting the content of recombinant human erythropoietin, which comprises the following steps: mixing a recombinant human erythropoietin physical and chemical reference substance with a solvent to prepare reference substances with different concentrations; taking a recombinant human erythropoietin sample, and preparing a to-be-detected product; performing high performance liquid chromatography detection on the reference substances with different concentrations, and establishing a corresponding relation between the concentration and the peak area; and performing high performance liquid chromatography detection on the to-be-detected product, substituting the obtained peak area into the corresponding relation between the concentration and the peak area, and calculating the content of the recombinant human erythropoietin in the to-be-detected product. The detection method is high in detection accuracy and simple to operate. 本发明涉及一种重组人促红细胞生成素的含量检测方法，包括如下步骤：将重组人促红细胞生成素理化对照品与溶剂