Application of high-flux real-time fluorescence detection and analysis system FLIPR in plant calcium signal detection

The invention provides the application of a high-throughput real-time fluorescence detection analysis system FLIPR in plant calcium signal detection, the FLIPR system is successfully applied to plant calcium signal detection, and the application of the FLIPR system in plant calcium signal detection...

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Hauptverfasser:	GONG JIYI, LI YUKE, OTOHIKI, LIU JIE, TANG MING, ZHANG XIMIN, ZHANG YUBIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING
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Beschreibung
Zusammenfassung:	The invention provides the application of a high-throughput real-time fluorescence detection analysis system FLIPR in plant calcium signal detection, the FLIPR system is successfully applied to plant calcium signal detection, and the application of the FLIPR system in plant calcium signal detection is increased. Meanwhile, the method for detecting the plant calcium signal based on FLIPR provided by the invention can detect the calcium signal change generated when the plant is stimulated by an exogenous calcium signal CaCl2 solution, and lays a foundation for realizing high-flux real-time fluorescence detection of plant calcium ions. 本发明提供了高通量实时荧光检测分析系统FLIPR在植物钙信号检测中的应用，将FLIPR系统成功地应用到了植物钙信号的检测中，增加了FLIPR系统在植物钙信号检测中的用途。同时，本发明提供的基于FLIPR检测植物钙信号的方法，对外源钙信号CaCl2溶液刺激植物所产生的钙信号变化进行检测，为实现植物钙离子高通量实时荧光检测奠定了基础。