IiPCR kit for identifying primary pathogen of porcine respiratory disease syndrome and use method of iiPCR kit

The invention relates to the technical field of animal virology, in particular to an iiPCR kit for identifying primary pathogens of porcine respiratory disease syndromes and a using method of the iiPCR kit, the kit comprises iiPCR amplification liquid, positive control and negative control which are independently configured for the four pathogens, the iiPCR amplification liquid comprises a Premix buffer solution, Taq DNA polymerase, MMLV reverse enzyme, a primer probe set and the like, and the primer probe set is used for detecting the primary pathogens of the porcine respiratory disease syndromes. The primer probe set comprises a first primer pair and a first probe, a second primer pair and a second probe, a third primer pair and a third probe, and a fourth primer pair and a fourth probe. For clinical samples such as lymph nodes, lungs and nasal swabs of pigs, through nucleic acid extraction, DNA or RNA can be directly used as a template, and iiPCR detection can be completed. 本发明涉及动物病毒学技术领域，具体涉及一种鉴别猪呼吸道疾病综合征