In-vitro three-dimensional cell or organoid culture device and culture system thereof

The invention discloses an in-vitro three-dimensional cell or organoid culture device, which comprises a substrate, a central assembly and a top cover, the substrate is provided with a liquid outlet, the top cover is provided with a liquid inlet, the top cover is detachably installed on the substrate, the central assembly is placed in the substrate, the central assembly comprises a bottom plate and a plurality of pore plate structures, the pore plate structures are movably installed on the bottom plate, and the bottom plate is provided with a liquid inlet. Therefore, the number of the pore plate structures can be changed, and the pore plate is used for culturing in-vitro three-dimensional cells or organoids and can meet the culture requirements of different in-vitro three-dimensional cells or organoids. The device overcomes the defect of low applicability of a device which can only meet the culture condition of a certain cell or organoid, and has the advantages that the number of pore plate structures for cul