Immobilized FAD synthetase and method for catalytically preparing flavin adenine dinucleotide

The invention discloses an immobilized FAD synthetase and a method for catalytically preparing flavin adenine dinucleotide by using the immobilized FAD synthetase, and discloses an FAD synthetase mutant E263A, and the amino acid sequence of the FAD synthetase mutant E263A is as shown in SEQ ID NO. 2...

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Bibliographische Detailangaben
Hauptverfasser:	JIA GUANYA, WANG JING, GAN HAISHENG, WANG JUN, NIU WEINING, LI HENG, ZHU ZHILONG, WANG YANXIONG, SONG XIANGYANG, LI ZE
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses an immobilized FAD synthetase and a method for catalytically preparing flavin adenine dinucleotide by using the immobilized FAD synthetase, and discloses an FAD synthetase mutant E263A, and the amino acid sequence of the FAD synthetase mutant E263A is as shown in SEQ ID NO. 2. The mutant with improved thermal stability is obtained, the stability of the mutant is improved, FAD can be catalytically synthesized in vitro, and the prepared product FAD is high in concentration, few in impurity and easy to purify. 本发明公开了一种固定化FAD合成酶及用于催化制备黄素腺嘌呤二核苷酸的方法，公开了一种FAD合成酶突变体E263A，其氨基酸序列为SEQ ID NO.2所示。本发明中得到了热稳定性提高的突变体，提高了其稳定性，可进行体外催化合成FAD，制备的产物FAD浓度高、杂质少，容易纯化。