Double-layer pig intestinal chip, pig artificial intestinal system and manufacturing and application thereof

The invention discloses a double-layer pig intestinal chip, a pig artificial intestinal system and manufacturing and application of the double-layer pig intestinal chip and the pig artificial intestinal system. The device comprises an upper-layer chip, a middle porous membrane and a lower-layer chip, wherein the upper-layer chip and the lower-layer chip are respectively provided with a bent intestine-shaped channel and a lateral electrode channel; the pig artificial intestinal system is composed of a double-layer pig intestinal chip, a bubble removing device, a fluid filling device and a connecting pipeline. Pig intestinal tract crypts are extracted and subjected to three-dimensional culture to obtain pig intestinal tract organoid, and then the pig intestinal tract organoid is cultured on the double-layer chip porous membrane. Through continuous perfusion of the culture medium, the pig artificial intestinal system which contains all terminally differentiated cell types of pig intestinal epithelium and has a t