Preparation method of recombinant A-type botulinum toxin

The invention relates to a preparation method of recombinant A-type botulinum toxin. The method comprises the following steps: preparing by adopting a gene recombination method, and constructing genetically engineered bacteria for respectively expressing light-chain protein and heavy-chain protein; carrying out first denaturation treatment on the light chain protein to obtain a first denaturation product; performing second denaturation treatment on the heavy-chain protein to obtain a second denaturation product; mixing the first denatured product and the second denatured product in proportion, and performing renaturation and assembly treatment to obtain the A-type botulinum toxin. The method disclosed by the invention is simple in process and short in preparation flow, and the prepared A-type botulinum toxin is high in purity and high in toxicity. 本发明涉及一种重组A型肉毒素的制备方法。所述方法包括：采用基因重组的方法进行制备，构建分别表达轻链蛋白、重链蛋白的基因工程菌；将轻链蛋白进行第一变性处理，得到第一变性产物；将重链蛋白进行第二变性处理，得到第二变性产物；将所述第一变性产物和第二变性产物按比例混合，进行复性及组装处理，获得所述A型肉毒素。本发明的方法工艺简单、制备