T cell death related gene 8 (TDAG8) modulation to enhance cellular cancer therapy

Embodiments of the present disclosure encompass improvement of cell therapy by allowing cells to be more effectively used in cancer therapy, including in solid tumor microenvironments. In particular cases, the cells are modified to have reduced or inhibited T cell death related gene 8 (TDAG8) expression levels, such as by CRISPR gene editing. In particular cases, the cell is further modified to express, for example, one or more engineered receptors, one or more cytokines, and optionally a suicide gene. 本公开的实施方案涵盖通过允许细胞更有效地用于癌症治疗(包括在实体瘤微环境中)来改善细胞疗法。在特定情况下，例如通过CRISPR基因编辑，修饰细胞以具有降低或抑制的T细胞死亡相关基因8(TDAG8)表达水平。在特定情况下，进一步修饰细胞以表达例如一种或多种工程化改造的受体、一种或多种细胞因子和任选的自杀基因。