Method for distinguishing outcrossing rate soybean male sterile line by dCAPS marker

The method for distinguishing the soybean male sterile line with the outcrossing rate by the dCAPS marker is used for identifying the high or low outcrossing rate of the soybean cytoplasmic male sterile line, and a convenient and fast way is provided for widening soybean CMS germplasm resources and identifying the sterile line with the high outcrossing rate and the low outcrossing rate. Differential SNP is found from a sterile line with a high outcrossing rate and a sterile line with a low outcrossing rate, a primer is designed in a flanking sequence of a cross-SNP region for PCR amplification, the amplified product fragment size can be used for distinguishing the male sterile line with the high outcrossing rate from the sterile line with the low outcrossing rate through restriction enzyme digestion, the strip is clear, the repeatability is good, the reliability is high, and the method is suitable for the large-scale production of the male sterile line with the high outcrossing rate and the small-scale produc