Kit for high-throughput determination of double-stranded DNA concentration and application thereof

The invention discloses a kit for high-throughput determination of double-stranded DNA concentration and application thereof. The kit comprises a dsDNA dye, a microwell plate and a plate sealing film, the dsDNA dye is sub-packaged in micropores in the microwell plate; and the plate sealing film comp...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	DONG JUNSHUAI, SONG GUIXUAN, CHANG GUORONG, ZHAI CHENGUANG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a kit for high-throughput determination of double-stranded DNA concentration and application thereof. The kit comprises a dsDNA dye, a microwell plate and a plate sealing film, the dsDNA dye is sub-packaged in micropores in the microwell plate; and the plate sealing film completely seals micropores in the microporous plate. The kit provided by the invention is applied to determination of dsDNA concentration. A method for measuring the concentration of the high-throughput dsDNA sample by adopting the kit comprises the following steps: 1) tearing off the sealing plate film of the kit; (2) respectively adding a dsDNA sample to be detected and dsDNA with known concentration into the dsDNA dye of the kit; 3) putting the microplate added with the dsDNA in the step 2) into a microplate reader for fluorescence detection to obtain fluorescence signals of the sample to be detected and the dsDNA with known concentration; and calculating the concentration of the dsDNA in the sample to be detected