Application of regulatory factor in improving content of polyunsaturated fatty acid of oleaginous microorganism and preparation method of microbial oil

The invention relates to the field of fermentation, and discloses an application of a regulatory factor in improving the content of polyunsaturated fatty acids of oleaginous microorganisms and a preparation method of microbial oil. The method for increasing the polyunsaturated fatty acid content of the oleaginous microorganisms comprises the following steps: inoculating the oleaginous microorganisms into a culture medium containing regulatory factors for culturing; the regulatory factor contains graphene oxide. The preparation method of the microbial oil comprises the following steps: culturing oil-producing microorganisms by adopting the method to obtain a culture solution, and carrying out wall breaking and extraction on the culture solution. The method provided by the invention can increase the content of polyunsaturated fatty acid in the oleaginous microorganisms, and is simple, efficient and low in cost. 本发明涉及发酵领域，公开了一种调节因子在提高产油微生物的多不饱和脂肪酸含量中的应用和微生物油脂的制备方法。提高产油微生物的多不饱和脂肪酸含量的方法包括：将产油微生物接入含有调节因子的培养基中进行培养；所