Construction method and application of 293T cell strain for improving virus packaging yield

The invention relates to a construction method and application of a 293T cell strain for improving virus packaging yield, and belongs to the technical field of biology.The construction method comprises the following steps that a target sequence of a targeted Teethrin gene is designed and synthesized, and Teethrin-sgRNACRISPR gene editing plasmids are constructed; a Teetherin-sgRNACRISPR gene is utilized to edit a plasmid to transfect a 293T cell; the method comprises the following steps: screening puromycin to obtain a Tethrin gene knockout monoclonal cell strain; packaging the virus; according to the 293T-Teetherin-KO cell obtained by the method, when the same amount of virus packaging plasmids are transfected, the quantity of produced viruses is higher than that of wild type 293T cells before treatment, namely, the expression of Teetherin genes is knocked out in the 293T cells, so that the yield of the viruses can be increased, and the production cost of the viruses is reduced. 本发明涉及一种提高病毒包装产量的293T细胞株构建方法及应