Reagent for transient expression transfection of HEK293 cells and transient expression system transfection method

The invention provides a reagent for transient expression transfection of HEK293 cells and a transient expression system transfection method, and relates to the technical field of genetic engineering, the reagent comprises a mixture of cell-penetrating peptide DLR8 and lipidosome, and the mass ratio of the cell-penetrating peptide DLR8 to the lipidosome in the reagent is (4-8.8): 1. In the HEK293 cell transient expression transfection reagent and the transient expression system transfection method, a mixture of cell-penetrating peptide DLR8 and lipidosome is selected as the HEK293 cell transient expression transfection reagent, a high-glucose DMEM culture medium is used as a transfection incubation Buffer, cell suspension is carried out 48 hours after cell transfection treatment, a small molecule additive sodium butyrate is added, and the transfection system transfection method is carried out. The expression level of the HEK293 cell foreign protein is improved, so that the target gene can be efficiently expre