Construction method of endo-beta-glucanase suitable for high-temperature granulation of feed

The invention discloses a construction method of endo-beta-glucanase suitable for feed high-temperature granulation, and belongs to the technical field of genetic engineering. The invention discloses a construction method of endo-beta-glucanase suitable for high-temperature granulation of feed. The construction method comprises the following steps: amplifying a target gene; carrying out enzyme digestion, connection and transformation; iPTG is utilized to induce expression of a target gene, and a protein crude enzyme solution is obtained through ultrasonication; and purifying the protein crude enzyme liquid by adopting a Ni-NTA purification medium. The beta-glucanase with high thermal stability is obtained through directed evolution, the specific enzyme activity is 91.6 U/mg, the half-life period at 90 DEG C is 1.5 h, and the beta-glucanase has huge potential in application in the field of feed. 本发明公开了一种适合饲料高温制粒的内切β-葡聚糖酶的构建方法，属于基因工程技术领域。本发明公开的一种适合饲料高温制粒的内切β-葡聚糖酶的构建方法，包括目的基因扩增；酶切、连接和转化；利用IPTG诱导目的基因的表达，经超声破碎获得蛋白