Preservation method of bacillus coagulans

The invention provides a preservation method of bacillus coagulans, which comprises the following steps: preparing an inclined plane by using an improved MRS culture medium, and carrying out air culture at 37 DEG C for 24 hours for later use. Selecting a bacillus coagulans glycerol bacterial liquid by using an inoculating loop, and scribing on the improved MRS inclined plane from bottom to top; the lineation slope is placed in a constant-temperature incubator at 37-42 DEG C to be cultured for 48-72 h; and taking out the inclined plane, scraping a ring by using a sterilized bamboo stick, inoculating into a sterilized sandy soil pipe, and storing in a refrigerator at 4 DEG C or-20 DEG C. After being cultured by the method, the thalli can be stored for a long time, are convenient to take and use, can be repeatedly used, and are convenient for industrial production. The vigor is not affected when being stored at 4 DEG C for 2 years and stored at-20 DEG C for 3-5 years. The strain still has very high activity afte