Lysosome targeting fluorescent probe as well as preparation method and application thereof

The invention belongs to the technical field of biology, and particularly relates to a lysosome targeting fluorescent probe as well as a preparation method and application thereof, in particular to an acidic organelle dye in a living cell. The positions of emission peaks of most of lysosome probes sold in the market at present are generally in the range of blue light and yellow light, fluorescent antibodies or other functional fluorescent dyes generally appear in the wave band, autofluorescence in cells is generally located in a blue-green area, and if a fluorescent indicator is located in the wave band or a nearby ultraviolet area, the wave bands can be mutually overlapped, imaging is affected, and imaging cannot be influenced. In order to solve the problem, the invention develops a lysosomal dye in a red spectral region, and particularly, the lysosomal dye can specifically mark and track acidic organelles in living cells at a lower nanomolar concentration. 本发明属于生物技术领域，具体涉及一种溶酶体靶向荧光探针及其制备方法和应用，为一种活细胞内酸性细胞器染料