Kaihoujian spray in-vivo plasma pharmaceutical chemical component and confirmation method thereof

The invention relates to in-vivo plasma pharmaceutical chemical components of Kaihoujian spray and a confirmation method thereof. On one hand, the invention relates to a method for confirming the in-vivo active pharmaceutical chemical components of Kaihoujian spray, which comprises the following operations: providing a high-resolution mass spectrometer and a liquid chromatograph, chromatographic conditions and mass spectrum conditions, administration and blood collection of experimental animals, treatment of a plasma sample, and analysis of the plasma sample. Absorption blood prototype medicine components and metabolism components are determined from various kinds of plasma, and according to the absorption blood prototype medicine common in various kinds of plasma, it is confirmed that the common absorption blood prototype medicine is the plasma active medicine chemical component, and the confirmed plasma active medicine chemical component is trifoliate pterocarpin. The method also comprises the step of confi