Deep eutectic solvent based on polyol hydrogen bond donor and method for improving catalytic activity of omega-transaminase by using deep eutectic solvent

The invention discloses a eutecticevaporate solvent based on a polyol hydrogen bond donor and a method for improving the catalytic activity of omega-transaminase, and particularly belongs to the technical field of catalysts.The method comprises the following steps that S01, a hydrogen bond donor rea...

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Hauptverfasser: WANG HONGPENG, CAO JIAREN, TAO YACHEN, HUANG JUN, XIAO ZHUQIAN, YANG JIAYAO
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a eutecticevaporate solvent based on a polyol hydrogen bond donor and a method for improving the catalytic activity of omega-transaminase, and particularly belongs to the technical field of catalysts.The method comprises the following steps that S01, a hydrogen bond donor reagent and a hydrogen bond acceptor reagent are mixed according to a preset proportion and heated to 70-80 DEG C, and a mixture is obtained; and reacting for 3-8 hours until a clear solution state is achieved, so as to obtain the eutectic solvent. And S02, mixing the deep-eutectic solvent with the buffer solution, so as to prepare the DES-buffer solution. The eutecticevaporate solvent prepared by the method can promote the dissolution of a substrate to a great extent. In the process of the enzymatic reaction of the omega-transaminase, the problem of low catalytic efficiency caused by poor solubility of a reaction substrate during the enzymatic reaction is solved, and the catalytic activity, thermal stability, preserv