Method for evaluating efficacy of swine mycoplasmal pneumonia inactivated vaccine

The invention provides a swine mycoplasmal pneumonia inactivated vaccine efficacy evaluation method based on guinea pig DTH. The method comprises the steps that after swine mycoplasmal pneumonia inactivated vaccine is subcutaneously injected into a guinea pig and immunity is enhanced, a swine mycoplasmal pneumonia antigen is injected to induce DTH reaction, the diameter of induration reaction is calculated according to the size of formed erythema, and then the vaccine efficacy is evaluated. Compared with an immune challenge method and an IHA method, the method provided by the invention has the advantages of low cost and simple operation, can well reflect the cellular immune response level after vaccine immunization, and is a new method for evaluating the effect after swine mycoplasmal pneumonia vaccine immunization. 本发明提供了一种基于豚鼠DTH的猪支原体肺炎灭活疫苗效力评估方法，包括向豚鼠皮下注射猪支原体肺炎灭活疫苗并加强免疫后，注射猪肺炎支原体抗原诱导DTH反应，根据形成的红斑大小计算硬结反应直径，进而评估疫苗效力的步骤。本发明相比于免疫攻毒法、IHA法，成本低廉、操作简便、能很好的反应疫苗免疫后的细胞免疫应答水平，是一种针对猪支原体肺炎疫苗免疫后效果评估的新方法。