Preparation method and application of platelet-rich plasma source exosome product

The invention relates to the technical field of biological medicines, in particular to the field of stem cells, and particularly relates to a preparation method and application of a platelet-rich plasma source exosome product. The preparation method of the platelet-rich plasma source exosome product...

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Bibliographische Detailangaben
Hauptverfasser: YANG HUILIN, GUO JIONGJIONG, ZHAO LINGYING, WU KAILUN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical field of biological medicines, in particular to the field of stem cells, and particularly relates to a preparation method and application of a platelet-rich plasma source exosome product. The preparation method of the platelet-rich plasma source exosome product comprises the following steps: S1, adding a mixed solution of a blood sample and a citric acid and gluconic acid solution A into an anticoagulant of the citric acid and gluconic acid solution A, and centrifuging; s2, continuously centrifuging to obtain platelet particles, and re-suspending the platelet particles by using a PBS (Phosphate Buffer Solution); activating the re-suspended platelet particles, and performing centrifugal treatment to obtain exosomes; and S3, after ultrafiltration, carrying out ultracentrifugation so as to granulate the exosome. And S4, adding an excipient and ultrapure water into the granulated exosome in S3, mixing, filtering, sterilizing, and freeze-drying to obtain a platelet-rich plasm