Food-borne melanoidin chelate nano-enzyme as well as preparation method and application thereof
The invention provides a food-borne melanoidin chelate nano-enzyme as well as a preparation method and application thereof, and belongs to the technical field of mimic enzymes and detection and analysis. According to the invention, food-borne melanoidin is adopted as a raw material, a chelate formed...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention provides a food-borne melanoidin chelate nano-enzyme as well as a preparation method and application thereof, and belongs to the technical field of mimic enzymes and detection and analysis. According to the invention, food-borne melanoidin is adopted as a raw material, a chelate formed by melanoidin and metal ions is synthesized, and it is determined that the chelate has peroxidase-like activity and can catalyze hydrogen peroxide to oxidize a chromogenic substrate for color development. The oxidized chromogenic substrate can be reduced by the antioxidant, so that the absorbance value of the system before and after the reduction reaction is determined, the absorbance difference value is calculated, a linear equation between the absorbance difference value and the concentration of the antioxidant is established, and the concentration of the antioxidant is determined after the absorbance value of the sample system to be detected is determined. The concentration of the antioxidant in the to-be-detec |
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