Tissue culture medium of Bo-chrysanthemum and tissue culture method thereof

The invention provides a Bo-chrysanthemum tissue culture medium and a tissue culture method thereof. The Bo-chrysanthemum tissue culture medium comprises a primary culture medium, a proliferation culture medium and a rooting culture medium, 6-BA with the concentration of 2.0 mg/L, NAA with the conce...

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Bibliographische Detailangaben
Hauptverfasser: ZHAO MENGRU, NIU YANYAN, LIU KAI, CHEN QINGQING, HUANG JUFEI, WU PENG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a Bo-chrysanthemum tissue culture medium and a tissue culture method thereof. The Bo-chrysanthemum tissue culture medium comprises a primary culture medium, a proliferation culture medium and a rooting culture medium, 6-BA with the concentration of 2.0 mg/L, NAA with the concentration of 0.2 mg/L, cane sugar with the concentration of 25 g/L and agar powder with the concentration of 7.5 g/L are added on the basis of the MS culture medium; proliferation culture medium: 6-BA with the concentration of 2.0 mg/L, NAA with the concentration of 0.2 mg/L, sucrose with the concentration of 25 g/L and L agar powder with the concentration of 7.5 g/L are added on the basis of the MS culture medium; a rooting medium; 0.03 mg/L of IBA, 0.01 mg/L of NAA, 40 g/L of cane sugar, 3 g/L of activated carbon and 6 g/L of agar powder are added on the basis of the 1/2 MS culture medium. Compared with the prior art, the Bo-chrysanthemum tissue culture medium and the tissue culture method thereof provided by the