Molecular marker of rice nitrogen efficient utilization gene OsTCP19 as well as identification method and application of molecular marker
The invention discloses a molecular marker of a rice nitrogen efficient utilization gene OsTCP19 and application of the molecular marker in rice breeding. The invention also discloses a molecular marker identification method of the rice nitrogen efficient utilization gene OsTCP19. The molecular mark...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a molecular marker of a rice nitrogen efficient utilization gene OsTCP19 and application of the molecular marker in rice breeding. The invention also discloses a molecular marker identification method of the rice nitrogen efficient utilization gene OsTCP19. The molecular marker provided by the invention is a functional molecular marker designed based on the DNA sequence of the gene, can directly and accurately reflect the genotype of the rice plant, and does not generate genotype identification errors caused by genetic exchange of similar linkage markers and genes; compared with sequencing identification, the molecular marker identification method disclosed by the invention has the advantages that the genotype of the OsTCP19 gene is identified by adopting a two-primer same-tube one-step PCR amplification method, so that the gene type of the OsTCP19 gene capable of efficiently and accurately utilizing the nitrogen in the rice can be efficiently and accurately identified, and the homozyg |
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