CPA primer combination and method for detecting shiga toxin-producing Escherichia coli

The invention discloses a cross primer nucleic acid isothermal amplification primer combination and method for detecting shiga toxin-producing Escherichia coli. The primer combination is used for detecting ten gene subtypes, namely stx1a, stx1c, stx1d, stx2a, stx2b, stx2c, stx2d, stx2e, stx2f and st...

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Hauptverfasser:	LIU YAN, FAN ZHIYONG, ZHOU QIAN, LIN YIZHI, PENG QINGZHI, ZHOU TAOHONG, WANG ZUNHAO, LI SHIYAO, YU ZHUJUN, ZANG WEIQING, YU JUNWEI, YOU QIMIN, ZHOU YANQIONG, ZHANG LI, WANG MINGQIU
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a cross primer nucleic acid isothermal amplification primer combination and method for detecting shiga toxin-producing Escherichia coli. The primer combination is used for detecting ten gene subtypes, namely stx1a, stx1c, stx1d, stx2a, stx2b, stx2c, stx2d, stx2e, stx2f and stx2g, and comprises a primer group I with nucleotide sequences as shown in SEQ ID NO: 1-5, a primer group II with nucleotide sequences as shown in SEQ ID NO: 6-10 and a primer group III with nucleotide sequences as shown in SEQ ID NO: 11-20. The method provided by the invention is simple to operate, good in specificity and high in sensitivity, nucleic acid detection can be completed within 50 minutes, and a rapid screening result is provided for supervision departments to guarantee major activities and detect food pathogenic bacteria in emergencies. Meanwhile, the primer combination is designed and screened for the first time to realize detection of all gene subtypes of STEC, and by combining the nucleic acid detect