Two-dimensional multiple gene mutation detection method

The invention relates to a two-dimensional multiple gene mutation detection method, and belongs to the technical field of molecular biology. The invention provides a two-dimensional multiple gene mutation detection method, a Blocker primer, an ARMS primer and a probe are simultaneously used for perf...

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Bibliographische Detailangaben
Hauptverfasser:	LI CHAO, ZHOU LIANQUN, GUO ZHEN, ZHOU HENG, LI JINZE, ZHANG ZHIQI, LI CHUANYU, TAO MINGLI, YAO JIA, ZHANG WEI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a two-dimensional multiple gene mutation detection method, and belongs to the technical field of molecular biology. The invention provides a two-dimensional multiple gene mutation detection method, a Blocker primer, an ARMS primer and a probe are simultaneously used for performing qPCR on a sample to be detected, the Blocker primer takes a wild type as a target, and the ARMS primer takes a mutant type as a target and comprises an upstream primer and a downstream primer; the 5'ends of the upstream primer and the downstream primer are connected with tail sequences, and the 3 'ends of the Blocker primer and the upstream primer are provided with 6-14 repeated basic groups. According to the primer design of the method, a Blocker primer and a wild type template are preferentially combined, so that combination of an ARMS primer and the wild type template is blocked, amplification of wild type alleles is inhibited, the ARMS primer and a mutant type template are combined, mutant type alleles a