Primer probe composition, kit and detection method for detecting EGFR (epidermal growth factor receptor) 19del based on digital PCR (polymerase chain reaction)

The invention discloses a primer probe composition, a kit and a detection method for detecting EGFR (Epidermal Growth Factor Receptor) 19del based on digital PCR (Polymerase Chain Reaction). The primer probe composition comprises a pair of 19del site specific primers and a pair of competitive binding probes, and can realize qualitative and quantitative detection of gene mutation on a ddPCR instrument. The primer probe composition is used for digital PCR detection of EGFR 19del, the method is easy and convenient to operate, the result is accurate and visual, and the primer probe composition can be applied to detection of samples such as tissues and cfDNA and has very high sensitivity and specificity. 本发明公开了一种基于数字PCR检测EGFR 19del的引物探针组合物、试剂盒和检测方法。所述引物探针组合物包括一对19del位点特异性引物和一对竞争性结合探针，其能够在ddPCR仪上实现基因突变定性定量检测。本发明引物探针组合物用于数字PCR检测EGFR 19del，方法操作简便，结果准确直观，可应用于组织和cfDNA等样本的检测，具有很高的灵敏度和特异度。