Descaled virus detection system, primer, probe and method
The invention provides a descaled virus detection system, primer, probe and method. The nucleotide sequence of the primer is shown as SEQ ID NO. 1 and SEQ ID NO. 2, and the nucleotide sequence of the probe is shown as SEQ ID NO. 3. The descaled virus detection system comprises the primer and/or the...
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| creator | YU FANGZHAO SU HUIBING WEN YANDONG MAO CAN PAN RUNHONG GU QUNHONG LI YONG LI WANGDONG GUO JIANYI |
| description | The invention provides a descaled virus detection system, primer, probe and method. The nucleotide sequence of the primer is shown as SEQ ID NO. 1 and SEQ ID NO. 2, and the nucleotide sequence of the probe is shown as SEQ ID NO. 3. The descaled virus detection system comprises the primer and/or the probe. The desquamation virus detection method adopts a micro-droplet digital PCR detection method, namely, after a tissue sample of to-be-detected fish is collected, DNA of the sample is extracted, and then whether the to-be-detected fish is infected or carries SDDV can be accurately detected by adopting micro-droplet digital PCR with the DNA as a template. The method has a good application prospect in selection of high-quality parents of cultured fishes.
本发明提供了脱鳞病毒检测体系、引物、探针及方法。所述引物的核苷酸序列如SEQ ID NO.1和SEQ ID NO.2所示，所述探针的核苷酸序列如SEQ ID NO.3所示。所述脱鳞病毒检测体系包括所述引物和/或探针。所述脱鳞病毒检测方法采用微滴式数字PCR检测方法，即采集待测鱼类的组织样本后，提取样本的DNA，然后以DNA为模板采用微滴式数字PCR，可准确检测出待测鱼类是否感染或携带SDDV，本发明的微滴式数字PCR检测方法灵敏度高，在养殖鱼类优质亲本选择中具有较好的应用前景。 |
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本发明提供了脱鳞病毒检测体系、引物、探针及方法。所述引物的核苷酸序列如SEQ ID NO.1和SEQ ID NO.2所示，所述探针的核苷酸序列如SEQ ID NO.3所示。所述脱鳞病毒检测体系包括所述引物和/或探针。所述脱鳞病毒检测方法采用微滴式数字PCR检测方法，即采集待测鱼类的组织样本后，提取样本的DNA，然后以DNA为模板采用微滴式数字PCR，可准确检测出待测鱼类是否感染或携带SDDV，本发明的微滴式数字PCR检测方法灵敏度高，在养殖鱼类优质亲本选择中具有较好的应用前景。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220812&DB=EPODOC&CC=CN&NR=114891926A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220812&DB=EPODOC&CC=CN&NR=114891926A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>YU FANGZHAO</creatorcontrib><creatorcontrib>SU HUIBING</creatorcontrib><creatorcontrib>WEN YANDONG</creatorcontrib><creatorcontrib>MAO CAN</creatorcontrib><creatorcontrib>PAN RUNHONG</creatorcontrib><creatorcontrib>GU QUNHONG</creatorcontrib><creatorcontrib>LI YONG</creatorcontrib><creatorcontrib>LI WANGDONG</creatorcontrib><creatorcontrib>GUO JIANYI</creatorcontrib><title>Descaled virus detection system, primer, probe and method</title><description>The invention provides a descaled virus detection system, primer, probe and method. The nucleotide sequence of the primer is shown as SEQ ID NO. 1 and SEQ ID NO. 2, and the nucleotide sequence of the probe is shown as SEQ ID NO. 3. The descaled virus detection system comprises the primer and/or the probe. The desquamation virus detection method adopts a micro-droplet digital PCR detection method, namely, after a tissue sample of to-be-detected fish is collected, DNA of the sample is extracted, and then whether the to-be-detected fish is infected or carries SDDV can be accurately detected by adopting micro-droplet digital PCR with the DNA as a template. The method has a good application prospect in selection of high-quality parents of cultured fishes.
本发明提供了脱鳞病毒检测体系、引物、探针及方法。所述引物的核苷酸序列如SEQ ID NO.1和SEQ ID NO.2所示，所述探针的核苷酸序列如SEQ ID NO.3所示。所述脱鳞病毒检测体系包括所述引物和/或探针。所述脱鳞病毒检测方法采用微滴式数字PCR检测方法，即采集待测鱼类的组织样本后，提取样本的DNA，然后以DNA为模板采用微滴式数字PCR，可准确检测出待测鱼类是否感染或携带SDDV，本发明的微滴式数字PCR检测方法灵敏度高，在养殖鱼类优质亲本选择中具有较好的应用前景。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLB0SS1OTsxJTVEoyywqLVZISS1JTS7JzM9TKK4sLknN1VEoKMrMTS0C0flJqQqJeSkKuaklGfkpPAysaYk5xam8UJqbQdHNNcTZQze1ID8-tbggMTk1L7Uk3tnP0NDEwtLQ0sjM0ZgYNQApKC53</recordid><startdate>20220812</startdate><enddate>20220812</enddate><creator>YU FANGZHAO</creator><creator>SU HUIBING</creator><creator>WEN YANDONG</creator><creator>MAO CAN</creator><creator>PAN RUNHONG</creator><creator>GU QUNHONG</creator><creator>LI YONG</creator><creator>LI WANGDONG</creator><creator>GUO JIANYI</creator><scope>EVB</scope></search><sort><creationdate>20220812</creationdate><title>Descaled virus detection system, primer, probe and method</title><author>YU FANGZHAO ; SU HUIBING ; WEN YANDONG ; MAO CAN ; PAN RUNHONG ; GU QUNHONG ; LI YONG ; LI WANGDONG ; GUO JIANYI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN114891926A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>YU FANGZHAO</creatorcontrib><creatorcontrib>SU HUIBING</creatorcontrib><creatorcontrib>WEN YANDONG</creatorcontrib><creatorcontrib>MAO CAN</creatorcontrib><creatorcontrib>PAN RUNHONG</creatorcontrib><creatorcontrib>GU QUNHONG</creatorcontrib><creatorcontrib>LI YONG</creatorcontrib><creatorcontrib>LI WANGDONG</creatorcontrib><creatorcontrib>GUO JIANYI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YU FANGZHAO</au><au>SU HUIBING</au><au>WEN YANDONG</au><au>MAO CAN</au><au>PAN RUNHONG</au><au>GU QUNHONG</au><au>LI YONG</au><au>LI WANGDONG</au><au>GUO JIANYI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Descaled virus detection system, primer, probe and method</title><date>2022-08-12</date><risdate>2022</risdate><abstract>The invention provides a descaled virus detection system, primer, probe and method. The nucleotide sequence of the primer is shown as SEQ ID NO. 1 and SEQ ID NO. 2, and the nucleotide sequence of the probe is shown as SEQ ID NO. 3. The descaled virus detection system comprises the primer and/or the probe. The desquamation virus detection method adopts a micro-droplet digital PCR detection method, namely, after a tissue sample of to-be-detected fish is collected, DNA of the sample is extracted, and then whether the to-be-detected fish is infected or carries SDDV can be accurately detected by adopting micro-droplet digital PCR with the DNA as a template. The method has a good application prospect in selection of high-quality parents of cultured fishes.
本发明提供了脱鳞病毒检测体系、引物、探针及方法。所述引物的核苷酸序列如SEQ ID NO.1和SEQ ID NO.2所示，所述探针的核苷酸序列如SEQ ID NO.3所示。所述脱鳞病毒检测体系包括所述引物和/或探针。所述脱鳞病毒检测方法采用微滴式数字PCR检测方法，即采集待测鱼类的组织样本后，提取样本的DNA，然后以DNA为模板采用微滴式数字PCR，可准确检测出待测鱼类是否感染或携带SDDV，本发明的微滴式数字PCR检测方法灵敏度高，在养殖鱼类优质亲本选择中具有较好的应用前景。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Descaled virus detection system, primer, probe and method |
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