Descaled virus detection system, primer, probe and method

The invention provides a descaled virus detection system, primer, probe and method. The nucleotide sequence of the primer is shown as SEQ ID NO. 1 and SEQ ID NO. 2, and the nucleotide sequence of the probe is shown as SEQ ID NO. 3. The descaled virus detection system comprises the primer and/or the...

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Hauptverfasser: YU FANGZHAO, SU HUIBING, WEN YANDONG, MAO CAN, PAN RUNHONG, GU QUNHONG, LI YONG, LI WANGDONG, GUO JIANYI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a descaled virus detection system, primer, probe and method. The nucleotide sequence of the primer is shown as SEQ ID NO. 1 and SEQ ID NO. 2, and the nucleotide sequence of the probe is shown as SEQ ID NO. 3. The descaled virus detection system comprises the primer and/or the probe. The desquamation virus detection method adopts a micro-droplet digital PCR detection method, namely, after a tissue sample of to-be-detected fish is collected, DNA of the sample is extracted, and then whether the to-be-detected fish is infected or carries SDDV can be accurately detected by adopting micro-droplet digital PCR with the DNA as a template. The method has a good application prospect in selection of high-quality parents of cultured fishes. 本发明提供了脱鳞病毒检测体系、引物、探针及方法。所述引物的核苷酸序列如SEQ ID NO.1和SEQ ID NO.2所示,所述探针的核苷酸序列如SEQ ID NO.3所示。所述脱鳞病毒检测体系包括所述引物和/或探针。所述脱鳞病毒检测方法采用微滴式数字PCR检测方法,即采集待测鱼类的组织样本后,提取样本的DNA,然后以DNA为模板采用微滴式数字PCR,可准确检测出待测鱼类是否感染或携带SDDV,本发明的微滴式数字PCR检测方法灵敏度高,在养殖鱼类优质亲本选择中具有较好的应用前景。