Honey pomelo black spot pathogen intercropping conchosphaeria LAMP detection primer group, detection method and application
The invention discloses an LAMP (loop-mediated isothermal amplification) detection primer group for honey pomelo black spot pathogen intercropping conchosphaeria, a detection method and application of the LAMP detection primer group. The LAMP primer group for detecting honey pomelo black spot pathog...
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| creator | YAO JIN'AI HOU XIANGYU ZENG TIANBAO DAI RUIQING LAI BAOCHUN |
| description | The invention discloses an LAMP (loop-mediated isothermal amplification) detection primer group for honey pomelo black spot pathogen intercropping conchosphaeria, a detection method and application of the LAMP detection primer group. The LAMP primer group for detecting honey pomelo black spot pathogen intercropping conchosphaeria is composed of a pair of outer side primers F3/B3 and a pair of inner side primers FIP/BIP. The invention also provides an LAMP detection method for honey pomelo black spot pathogen intercropping conchosphaeria by using the primer. The LAMP detection method comprises the following specific steps: 1) extracting DNA of a sample to be detected; 2) establishment of an LAMP reaction system; and 3) result detection. The LAMP reaction system is carried out under the isothermal condition of 65 DEG C, and the honey pomelo black spot pathogen intercropping conchosphaeria can be rapidly, conveniently, efficiently, specifically and sensitively detected through 1h incubation. The method does not |
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The LAMP primer group for detecting honey pomelo black spot pathogen intercropping conchosphaeria is composed of a pair of outer side primers F3/B3 and a pair of inner side primers FIP/BIP. The invention also provides an LAMP detection method for honey pomelo black spot pathogen intercropping conchosphaeria by using the primer. The LAMP detection method comprises the following specific steps: 1) extracting DNA of a sample to be detected; 2) establishment of an LAMP reaction system; and 3) result detection. The LAMP reaction system is carried out under the isothermal condition of 65 DEG C, and the honey pomelo black spot pathogen intercropping conchosphaeria can be rapidly, conveniently, efficiently, specifically and sensitively detected through 1h incubation. 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The LAMP primer group for detecting honey pomelo black spot pathogen intercropping conchosphaeria is composed of a pair of outer side primers F3/B3 and a pair of inner side primers FIP/BIP. The invention also provides an LAMP detection method for honey pomelo black spot pathogen intercropping conchosphaeria by using the primer. The LAMP detection method comprises the following specific steps: 1) extracting DNA of a sample to be detected; 2) establishment of an LAMP reaction system; and 3) result detection. The LAMP reaction system is carried out under the isothermal condition of 65 DEG C, and the honey pomelo black spot pathogen intercropping conchosphaeria can be rapidly, conveniently, efficiently, specifically and sensitively detected through 1h incubation. The method does not</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Honey pomelo black spot pathogen intercropping conchosphaeria LAMP detection primer group, detection method and application |
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