Relative quantitative detection method for stomach tissue tRF-28-ZJ47D112Z4DZ and application thereof

The invention relates to a relative quantitative detection method for stomach tissue tRF-28-ZJ47D112Z4DZ and application of the relative quantitative detection method, and is characterized in that the nucleotide sequence of the tRF-28-ZJ47D112Z4DZ is shown as SEQ ID NO: 1, and the specific expressio...

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Hauptverfasser:	YU XIUCHONG, XIE YAOYAO, WENG QIUYAN, SONG XUEMEI, GUO JUNMING, ZHANG SHUANGSHUANG, YAN ZHILONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a relative quantitative detection method for stomach tissue tRF-28-ZJ47D112Z4DZ and application of the relative quantitative detection method, and is characterized in that the nucleotide sequence of the tRF-28-ZJ47D112Z4DZ is shown as SEQ ID NO: 1, and the specific expression of the tRF-28-ZJ47D112Z4DZ in the tissue of a gastric cancer patient is down-regulated: a primer is F1: 5 '-CTCCGGTTGGCTTCCTAAG-3'; r1: 5 '-GGTACCTCTCTCTCTCTCTCTCTAC-3'. Compared with the prior art, the invention has the advantage that the tRF-28-ZJ47D112Z4DZ can be used as a novel molecular marker for gastric cancer diagnosis when the specific expression of the tRF-28-ZJ47D112Z4DZ is down-regulated in gastric cancer tissues. 本发明涉及一种用于胃组织tRF-28-ZJ47D112Z4DZ的相对定量检测方法及其应用，其特征在于：该tRF-28-ZJ47D112Z4DZ的核苷酸序列如SEQ ID NO：1所示，该tRF-28-ZJ47D112Z4DZ在胃癌患者组织中的特异性表达下调：所述引物为：F1：5'-CTCCCGGTTGGCTTCCTAAG-3'；R1：5'-GGTACCTCCTCTCTTCTCTACT-3'，与现有技术相比，本发明的优点在于胃癌组织中的特异性表达下调tRF-28-ZJ47D112Z4DZ可作为胃癌诊断的新型分子标记物。