LAMP (loop-mediated isothermal amplification) method-based codling moth identification primer group, identification method and identification kit
The invention discloses a codling moth identification primer group based on an LAMP method, an identification method and an identification kit, and belongs to the technical field of agricultural pest identification. The codling moth identification primer group based on the LAMP method is designed ac...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a codling moth identification primer group based on an LAMP method, an identification method and an identification kit, and belongs to the technical field of agricultural pest identification. The codling moth identification primer group based on the LAMP method is designed according to a nucleotide sequence as shown in SEQ ID NO: 1; the method comprises the following steps: carrying out LAMP reaction by adopting the primer group and taking whole genome DNA or partial tissue of an insect to be detected as a template; after the reaction is finished, detecting whether an amplification product exists or not; if amplification exists, the to-be-detected insect is the codling moth. According to the invention, rapid molecular detection and a visualization method are used for identifying codling moths, and a detection result is obtained within 1 hour. The cover-opening-free visual detection method can effectively avoid aerosol pollution and accurately develop color, the color development result |
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