Classical swine fever virus detection method based on G4-ThT biosensor and NASBA and kit thereof

The invention belongs to the technical field of gene engineering, and particularly relates to a hog cholera virus detection method and kit based on a G4-ThT biosensor and NASBA, and the specific method comprises the following steps: taking virus RNA extracted from a hog cholera virus sample as an amplification object, carrying out isothermal amplification by adopting an isothermal nucleic acid amplification technology, then adding a buffer solution containing Tris-HCl and KCl, and carrying out isothermal amplification by adopting an isothermal nucleic acid amplification technology; then thioflavin T is added to promote G-quadruplex on an amplification product to be folded and form a compound, fluorescence is detected under the excitation condition of a light source with a specific wavelength, and judgment is carried out according to the intensity of the fluorescence. The invention provides a detection method and a kit which do not need special biotin labeling or sulfydryl modification treatment probes, and th