Mutant of short-chain dehydrogenase, encoding gene, encoding gene obtaining method and application of mutant

The invention belongs to the technical field of biological engineering, and particularly relates to a mutant of short-chain dehydrogenase, and the mutant is a mutant L104A, a mutant A150Y, a mutant Y155A, a mutant I158A, a mutant I202A or a mutant T205A. The amino acid sequence of the short-chain de...

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Hauptverfasser: XIA NANA, LI DONGYAN, YU HONGWEI
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LI DONGYAN
YU HONGWEI
description The invention belongs to the technical field of biological engineering, and particularly relates to a mutant of short-chain dehydrogenase, and the mutant is a mutant L104A, a mutant A150Y, a mutant Y155A, a mutant I158A, a mutant I202A or a mutant T205A. The amino acid sequence of the short-chain dehydrogenase is as shown in SEQ ID NO. 1. Compared with wild type short-chain dehydrogenase, the short-chain dehydrogenase mutant has higher enzyme activity, prednisolone can be prepared from prednisone or prednisone acetate matched with hydrolase as a substrate, the conversion rate of the substrate is high, the yield of the product is high, no by-product is generated, the reaction can be carried out at a higher substrate concentration level, and the method is suitable for industrial production. The method is suitable for large-scale production. 本发明属于生物工程技术领域,具体涉及一种短链脱氢酶的突变体,所述突变体为突变体L104A、突变体A150Y、突变体Y155A、突变体I158A、突变体I202A或者突变体T205A;所述短链脱氢酶的氨基酸序列如SEQ ID NO.1所示。本发明的短链脱氢酶突变体相对野生型短链脱氢酶有更高的酶活性,可以泼尼松或者配合水解酶以醋酸泼尼松为底物制备泼
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The amino acid sequence of the short-chain dehydrogenase is as shown in SEQ ID NO. 1. Compared with wild type short-chain dehydrogenase, the short-chain dehydrogenase mutant has higher enzyme activity, prednisolone can be prepared from prednisone or prednisone acetate matched with hydrolase as a substrate, the conversion rate of the substrate is high, the yield of the product is high, no by-product is generated, the reaction can be carried out at a higher substrate concentration level, and the method is suitable for industrial production. The method is suitable for large-scale production. 本发明属于生物工程技术领域,具体涉及一种短链脱氢酶的突变体,所述突变体为突变体L104A、突变体A150Y、突变体Y155A、突变体I158A、突变体I202A或者突变体T205A;所述短链脱氢酶的氨基酸序列如SEQ ID NO.1所示。本发明的短链脱氢酶突变体相对野生型短链脱氢酶有更高的酶活性,可以泼尼松或者配合水解酶以醋酸泼尼松为底物制备泼</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20220809&amp;DB=EPODOC&amp;CC=CN&amp;NR=114875003A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,778,883,25551,76302</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20220809&amp;DB=EPODOC&amp;CC=CN&amp;NR=114875003A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>XIA NANA</creatorcontrib><creatorcontrib>LI DONGYAN</creatorcontrib><creatorcontrib>YU HONGWEI</creatorcontrib><title>Mutant of short-chain dehydrogenase, encoding gene, encoding gene obtaining method and application of mutant</title><description>The invention belongs to the technical field of biological engineering, and particularly relates to a mutant of short-chain dehydrogenase, and the mutant is a mutant L104A, a mutant A150Y, a mutant Y155A, a mutant I158A, a mutant I202A or a mutant T205A. The amino acid sequence of the short-chain dehydrogenase is as shown in SEQ ID NO. 1. Compared with wild type short-chain dehydrogenase, the short-chain dehydrogenase mutant has higher enzyme activity, prednisolone can be prepared from prednisone or prednisone acetate matched with hydrolase as a substrate, the conversion rate of the substrate is high, the yield of the product is high, no by-product is generated, the reaction can be carried out at a higher substrate concentration level, and the method is suitable for industrial production. 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The amino acid sequence of the short-chain dehydrogenase is as shown in SEQ ID NO. 1. Compared with wild type short-chain dehydrogenase, the short-chain dehydrogenase mutant has higher enzyme activity, prednisolone can be prepared from prednisone or prednisone acetate matched with hydrolase as a substrate, the conversion rate of the substrate is high, the yield of the product is high, no by-product is generated, the reaction can be carried out at a higher substrate concentration level, and the method is suitable for industrial production. The method is suitable for large-scale production. 本发明属于生物工程技术领域,具体涉及一种短链脱氢酶的突变体,所述突变体为突变体L104A、突变体A150Y、突变体Y155A、突变体I158A、突变体I202A或者突变体T205A;所述短链脱氢酶的氨基酸序列如SEQ ID NO.1所示。本发明的短链脱氢酶突变体相对野生型短链脱氢酶有更高的酶活性,可以泼尼松或者配合水解酶以醋酸泼尼松为底物制备泼</abstract><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Mutant of short-chain dehydrogenase, encoding gene, encoding gene obtaining method and application of mutant
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