Mutant of short-chain dehydrogenase, encoding gene, encoding gene obtaining method and application of mutant

The invention belongs to the technical field of biological engineering, and particularly relates to a mutant of short-chain dehydrogenase, and the mutant is a mutant L104A, a mutant A150Y, a mutant Y155A, a mutant I158A, a mutant I202A or a mutant T205A. The amino acid sequence of the short-chain dehydrogenase is as shown in SEQ ID NO. 1. Compared with wild type short-chain dehydrogenase, the short-chain dehydrogenase mutant has higher enzyme activity, prednisolone can be prepared from prednisone or prednisone acetate matched with hydrolase as a substrate, the conversion rate of the substrate is high, the yield of the product is high, no by-product is generated, the reaction can be carried out at a higher substrate concentration level, and the method is suitable for industrial production. The method is suitable for large-scale production. 本发明属于生物工程技术领域，具体涉及一种短链脱氢酶的突变体，所述突变体为突变体L104A、突变体A150Y、突变体Y155A、突变体I158A、突变体I202A或者突变体T205A；所述短链脱氢酶的氨基酸序列如SEQ ID NO.1所示。本发明的短链脱氢酶突变体相对野生型短链脱氢酶有更高的酶活性，可以泼尼松或者配合水解酶以醋酸泼尼松为底物制备泼