Separation and purification of bacteriocin produced by lactobacillus sake, antibacterial application of bacteriocin produced by lactobacillus sake and used lactic acid bacteria
The invention discloses lactobacillus sake ZFM225, and the preservation number of the lactobacillus sake ZFM225 is CCTCC (China Center For Type Culture Collection) NO: M 2016669. The invention also discloses a separation and purification method of the lactobacillus sake ZFM225 bacteriocin, which com...
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| creator | LI PING YE PENGXIN GU QING ZHOU QINGQING SHENTU HUIFEI |
| description | The invention discloses lactobacillus sake ZFM225, and the preservation number of the lactobacillus sake ZFM225 is CCTCC (China Center For Type Culture Collection) NO: M 2016669. The invention also discloses a separation and purification method of the lactobacillus sake ZFM225 bacteriocin, which comprises the following steps of: precipitating fermentation supernate of the lactobacillus sake ZFM225 by using an ammonium sulfate precipitation method to obtain crude protein; desalting the crude protein to obtain a protein crude extract; carrying out cation exchange chromatography separation on the protein crude extract, eluting with a gradient washing solution containing 0.5-1.0 M NaCl, and carrying out desalination treatment on the obtained eluent to obtain a protein primary purification solution; and carrying out reversed-phase high-performance liquid chromatography purification to obtain the lactobacillus sake ZFM225 bacteriocin. The bacteriocin of the lactobacillus sake ZFM225 has antibacterial activity on bo |
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The invention also discloses a separation and purification method of the lactobacillus sake ZFM225 bacteriocin, which comprises the following steps of: precipitating fermentation supernate of the lactobacillus sake ZFM225 by using an ammonium sulfate precipitation method to obtain crude protein; desalting the crude protein to obtain a protein crude extract; carrying out cation exchange chromatography separation on the protein crude extract, eluting with a gradient washing solution containing 0.5-1.0 M NaCl, and carrying out desalination treatment on the obtained eluent to obtain a protein primary purification solution; and carrying out reversed-phase high-performance liquid chromatography purification to obtain the lactobacillus sake ZFM225 bacteriocin. 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The invention also discloses a separation and purification method of the lactobacillus sake ZFM225 bacteriocin, which comprises the following steps of: precipitating fermentation supernate of the lactobacillus sake ZFM225 by using an ammonium sulfate precipitation method to obtain crude protein; desalting the crude protein to obtain a protein crude extract; carrying out cation exchange chromatography separation on the protein crude extract, eluting with a gradient washing solution containing 0.5-1.0 M NaCl, and carrying out desalination treatment on the obtained eluent to obtain a protein primary purification solution; and carrying out reversed-phase high-performance liquid chromatography purification to obtain the lactobacillus sake ZFM225 bacteriocin. 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The invention also discloses a separation and purification method of the lactobacillus sake ZFM225 bacteriocin, which comprises the following steps of: precipitating fermentation supernate of the lactobacillus sake ZFM225 by using an ammonium sulfate precipitation method to obtain crude protein; desalting the crude protein to obtain a protein crude extract; carrying out cation exchange chromatography separation on the protein crude extract, eluting with a gradient washing solution containing 0.5-1.0 M NaCl, and carrying out desalination treatment on the obtained eluent to obtain a protein primary purification solution; and carrying out reversed-phase high-performance liquid chromatography purification to obtain the lactobacillus sake ZFM225 bacteriocin. The bacteriocin of the lactobacillus sake ZFM225 has antibacterial activity on bo</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE |
| title | Separation and purification of bacteriocin produced by lactobacillus sake, antibacterial application of bacteriocin produced by lactobacillus sake and used lactic acid bacteria |
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