Chromatographic separation method of deoxynucleotide and preparation of sodium deoxynucleotide bulk drug

The invention discloses a deoxynucleotide chromatographic separation method and a preparation method of a deoxynucleotide sodium bulk drug, and the method comprises the following steps: carrying out chromatographic separation on DNA enzymatic hydrolysate by adopting real-time online detection and intelligent control, monitoring whether deoxynucleotide flows out in a sample loading and water washing process or not through real-time online detection of a nucleic acid protein detector, and determining whether the deoxynucleotide flows out or not. Therefore, whether the process runs normally or not is known; controlling a segmented elution process and collecting four deoxynucleotide solutions of 5 '-dCMP, 5'-dAMP, 5 '-dTMP and 5'-dGMP through timed online sampling and HPLC (High Performance Liquid Chromatography) detector analysis; according to the present invention, the unmanned operation of the whole chromatographic separation process is achieved, the process control is precise and convenient, the time and the