Near-infrared & two-photon dynamics dual-mode high-stability fluorescent dye, NTR&HClO probe and synthesis method and application

The present invention relates to ROSamp; the invention belongs to the technical field of AOS detection, and particularly discloses a single-molecule dual-mode excitation probe and a synthesis process and application thereof. The structural formula of the single-molecule dual-mode excitation probe is shown in the specification. According to the invention, firstly, the structure of traditional cyanine is optimized, and a nitrobenzene benzyl structure compound CY-8 with a large light absorption coefficient is synthesized, so that the stability of a near-infrared frame (CY-8) is enhanced. Secondly, naphthol biphenyl alkene dinitrile is combined to form a two-photon response part (compound 2); and then connecting two parts of the CY-8 and the compound 2 to obtain the CYNA-CN. The compound has a dual-mode (near-infrared and two-photon) fluorescence excitation function, so that nitroreductase (NTR) and hypochlorous acid (HClO) in cancer cells can be independently detected in a non-interference excitation mode. 本发明涉及