Near-infrared fluorescent probe for detecting temperature as well as preparation method and application of near-infrared fluorescent probe

The invention aims to provide a high-sensitivity near-infrared fluorescent probe for detecting temperature as well as a preparation method and application of the high-sensitivity near-infrared fluorescent probe. The preparation method comprises the following steps: adding malononitrile and a compound (5) into an acetic acid and ammonium acetate solution, and after the reaction is finished, separating and purifying a reaction solution to obtain a compound (4); and adding the compound (3), the compound (2) and piperidine into an ethanol solution, and separating and purifying a reaction solution after reaction to obtain the compound (1). The temperature detection application is used for detecting the temperature of cells, and the change of fluorescence intensity is about 30 times when the detected temperature is between 25 DEG C and 60 DEG C. 本发明目的是提供一种可以用于检测温度的高灵敏度近红外荧光探针，及其制备方法与应用。其制备方法包括：将丙二腈和化合物(5)加入乙酸、乙酸铵溶液中，反应结束后反应液分离纯化得到化合物(4)；将化合物(3)、化合物(2)和哌啶加入乙醇溶液中，反应结束后反应液分离纯化得到化合物(1)。所述温度检测应用：用于检测细胞的温度，在所测温度25℃-60℃间，