IRAP molecular marker primer for identifying sand prunus salicina germplasm based on reverse transcription transposon and identification method thereof
The invention discloses an IRAP molecular marker primer for identifying sand prunus salicina germplasm based on a reverse transcriptase. The IRAP molecular marker primer is characterized in that the IRAP molecular marker primer is one or a mixture of more than one of Ty1-6, Ty1-7, Ty1-9, Ty1-15, Ty1...
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Zusammenfassung: | The invention discloses an IRAP molecular marker primer for identifying sand prunus salicina germplasm based on a reverse transcriptase. The IRAP molecular marker primer is characterized in that the IRAP molecular marker primer is one or a mixture of more than one of Ty1-6, Ty1-7, Ty1-9, Ty1-15, Ty1-18, Ty1-20, Ty1-25, Ty3-2, Ty3-3, Ty3-5, Ty3-6, Ty3-9, Ty3-14 and Ty3-16. According to the invention, detection is carried out from the molecular level based on the characteristics of the retrotransposon, and the result is more accurate and reliable; by detecting dozens of sand prunus salicina germplasm and repeating for multiple times, the detection results are kept consistent, and the results are accurate and reliable.
本发明公开了一种基于反转录转座子鉴定沙子空心李种质的IRAP分子标记引物,其特征在于:所述的IRAP分子标记引物为Ty1-6、Ty1-7、Ty1-9、Ty1-15、Ty1-18、Ty1-20、Ty1-25、Ty3-2、Ty3-3、Ty3-5、Ty3-6、Ty3-9、Ty3-14及Ty3-16中的一种或一种以上混合。本发明从分子水平基于反转录转座子的特性进行检测,结果更为准确可靠;通过对数十种沙子空心李种质进行检测,经多次重复,检测结果均保持一致,结果准确可靠。 |
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