Culture method for extracting immune cells from umbilical cord blood

The invention belongs to the technical field of cell biology, and particularly relates to a culture method for extracting immune cells from umbilical cord blood, which comprises the following steps: S1, culturing mononuclear cells of the umbilical cord blood by using a first culture medium until the cell growth state is stable, and subsequently culturing by using a second culture medium. According to the invention, for the same kind of immune cells, under the condition that the total use amount of the culture solution is not changed, the proportional relation between the volume of the culture solution and the volume of the culture bag is changed, and the amplification capacity and the number of the cells can be changed, so that the proportion of the volume of the culture solution and the volume of the culture bag is reduced, and the amplification capacity of the immune cells is improved to a certain extent; more immune cells can be obtained through the whole culture method, and later clinical use can be bette