Construction method and application of grass carp skeletal muscle myoblast line

The invention discloses a construction method and application of a grass carp skeletal muscle myoblast line. The preparation method comprises the following three main steps: primary culture, myoblast purification and subculture. An immortalized skeletal muscle myoblast line is established on the basis of muscle tissues on the back of the grass carp by adopting a collagenase and trypsin combination method, and confirmation is carried out through myogenic characteristic indexes and chromosome characteristic indexes of the grass carp. The cell line has good transfection performance, has remarkable growth performance difference under treatment of different arginines, and can be used for research work of regulation and control effects of specific gene functions and nutrient substances on grass carp muscle growth and differentiation, molecular mechanisms of the regulation and control effects and the like. 本发明公开了一种草鱼骨骼肌成肌细胞系的构建方法及其应用。本发明所述制备方法包括：原代培养、成肌细胞纯化和传代培养这三个主要步骤。本发明采用胶原蛋白酶和胰蛋白酶结合法基于草鱼背部的肌肉组织建立了一种永生化骨骼肌成肌细胞系，并