Suspension medium for suspension 3D biological printing and preparation method and application thereof

The invention belongs to the technical field of biological materials, and discloses a suspension medium for suspension 3D biological printing and a preparation method and application thereof. The suspension medium comprises but is not limited to the following components in percentage by mass: 0-10% of gellan gum, 0-10% of sulfhydrylated gellan gum and the balance of a solvent, the contents of the gellan gum and the sulfhydrylated gellan gum are not zero at the same time. The suspension medium disclosed by the invention has good thixotropy and self-repairing capability, so that a spray head of a 3D bio-printer can freely move in the suspension medium, and meanwhile, the integrity of a 3D bio-printing structure can be kept; the problem that in traditional printing, natural high polymer materials such as collagen and fibrous protein cannot achieve self-supporting printing is solved, the printing precision can reach the 200 micron level, the fidelity reaches 99%, and the 3D printing material is expected to be use