Method for purifying recombinant mussel-like mucin

The invention discloses a method for purifying recombinant mussel-like mucoprotein, and relates to the technical field of protein purification methods. According to the method for purifying the recombinant mussel-like mucin, the recombinant mussel-like mucin fermentation liquor which is subjected to...

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Bibliographische Detailangaben
Hauptverfasser: HOU ZENGMIAO, ZHAO SHUOWEN, WEI WENPEI, HAO DONG, QIAO SHA, ZHOU HAO, ZHANG LIFENG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for purifying recombinant mussel-like mucoprotein, and relates to the technical field of protein purification methods. According to the method for purifying the recombinant mussel-like mucin, the recombinant mussel-like mucin fermentation liquor which is subjected to fermentation induced expression of escherichia coli engineering bacteria is adopted as a raw material for the first time, and the method of removing impurities through cell disruption, enzyme catalytic oxidation, chromatographic purification and endotoxin removal through chromatography is adopted, so that the recombinant mussel-like mucin which the yield of each liter of fermentation liquor pure product can reach 0.7 g/L, the purity is greater than 95%, and L-3, 5-dihydroxy-2, 5-dihydroxy-2, 5-dihydroxy-2, 5-dihydroxy-2, 5-dihydroxy-2, 5-dihydroxy-2, 5- The method for purifying the recombinant mussel-like mucoprotein (Mlfp-151 with theoretical molecular weight of 22.61 KD) with the content of 2, 4-dihydroxyphenyla