Human exfoliated cell immunofluorescent staining kit and method

The invention discloses a fluorescent staining kit and method for human exfoliated cells, which comprises the following steps: staining cell nuclei and cell nuclei into green by using a fluorescent dye SYTO RNA Select with special affinity to the cell nuclei, especially RNA of the cell nuclei, and marking a red fluorescent dye by combining monoclonal antibodies of epithelial cell antigens and the like (EpCAM, CK, waveform protein and the like). And dyeing the cytoplasm and the cell surface into red. According to the staining method and the staining kit, the cell nucleus can be clearly and completely displayed, especially the nucleolus of the cell nucleus can be calibrated, and the number, the shape, the volume and the distribution of the nucleolus can be clearly seen. The method is a key characteristic for analyzing human body cell pathology, and an immunofluorescence staining method is used for characteristic display of antigens of cytoplasm and cell membranes, so that the human body exfoliated cells can be